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Detection of new double-membrane structures in native mitochondria by the method of small-angle neutron scattering |
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| Authors: | T. N. Murugova V. I. Gordeliy A. I. Kuklin Yu. S. Kovalev V. I. Yurkov A. Nurenberg A. Kh. Islamov L. S. Yaguzhinskii |
| Affiliation: | (1) Department of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119992, Russia;(2) Frank Laboratory of Neutron Physics, Joint Institute of Nuclear Research, Dubna, Moscow oblast, 141980, Russia;(3) IBI-2 Forschungszentrum Juelich, IBI-2, 52425, Germany;(4) Center of Biophysics and Physical Chemistry of Supramolecular Structures, Moscow Institute for Physics and Technology, Dolgoprudnyi, Moscow oblast, 141700, Russia;(5) Belozerskii Institute of Physicochemical Biology, Moscow State University, Moscow, 119899, Russia |
| Abstract: | The structure of mitochondrial cristae has been studied for the first time by the method of small-angle neutron scattering. Experiments were performed on intact (functioning) mitochondria from rat liver. Mitochondrial cristae are usually considered to be folds of the inner membrane with arbitrary variable intermembrane distances. Under conditions of low-amplitude swelling, mitochondrial cristae transformed into double-membrane structures with a distance of 190 Å between the central planes of the membranes. The formation of double-membrane structures and their structural parameters did not depend on the method for inducing swelling which was accomplished either by placing the mitochondria into a hypotonic medium or through the opening of nonspecific pores. |
| Keywords: | mitochondria osmotic pressure small-angle neutron scattering |
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