Plant regeneration from protoplasts ofCapsicum annuum L. cv. California Wonder |
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Authors: | A H Prakash K Sankara Rao M Udaya Kumar |
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Institution: | (1) Department of Biochemistry, Indian Institute of Science, 560012 Bangalore, India;(2) Central Institute for Cotton Research, P O Box No. 225, GPO, 440001 Nagpur, India;(3) Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065 Bangalore, India |
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Abstract: | Plant regeneration from mesophyll protoplasts of pepper,Capsicum annuum
L. cv. California Wonder has been demonstrated via shoot organogenesis. Protoplasts isolated from fully expanded leaves of
3-week-old axenic shoots when cultured in TM medium supplemented with 1 mg l
−1
NAA, 1 mg l
−1
2,4-D, 0 5 mg l
−1
BAP (CM 1) resulted in divisions with a frequency ranging from 20–25 %. Antioxidant ascorbic acid and polyvinylpyrrolidone
(PVP) in the medium and incubation in the dark helped overcome browning of protoplasts. Microcalli and macrocalli were formed
in TM medium containing 2 mg l
−1
NAA and 0.5 mg l
−1
BAP (CM II) and MS gelled medium containing 2 mg 1
−1
NAA and 0 5 mg 1
−1
BAP (CM III), respectively. Regeneration of plantlets was possible via caulogenesis. Microshoots, 2–5 percallus appeared on
MS gelled medium enriched with 0.5 mg l
−1
IAA, 2mg l
−1
GA and l0mg l
−1
BAP (CM IVc). Rooting of microshoots was obtained on half strength gelled medium containing 1 mg l
−1
NAA and 0.5mg l
−1
BAP. Protoplasts isolated from cotyledons failed to divide and degenerated eventually. |
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Keywords: | Leaf protoplasts Capsicum annuum L cv California Wonder regeneration organogenesis |
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