Assignment of a porcine male-specific DNA repeat to Y-chromosomal heterochromatin. |
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| Authors: | P D Thomsen J Hindkjaer K Christensen |
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| Institution: | Department of Animal Science and Animal Health, Royal Veterinary and Agricultural University, Copenhagen, Denmark. |
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| Abstract: | Primers were designed to amplify by PCR a 509-bp genomic fragment from male pig DNA, using the porcine male-specific repeat sequence described by McGraw et al. (1988). This PCR product showed male-specific hybridization in Southern blots. Nonradioactive in situ hybridization localized it to the entire length of the heterochromatic portion of Yq. The assignment was confirmed using the PCR primer pDYZ1-S for primed in situ labeling. |
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