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| 基于PCR的cDNA基因克隆技术研究进展 | |
| 引用本文： | 蔡欣,陈宏,汪虹英. 基于PCR的cDNA基因克隆技术研究进展[J]. 生物技术通讯, 2004, 15(6): 623-625 |
| 作者姓名： | 蔡欣 陈宏 汪虹英 |
| 作者单位： | 1. 西北农林科技大学,生命科学院,陕西省农业分子生物学重点实验室,杨凌,712100 2. 西北农林科技大学,动物科技学院,陕西省农业分子生物学重点实验室,杨凌,712100;徐州师范大学,生物技术研究所,徐州,221116 3. 甘肃省漳县一中,漳县,748300 |
| 基金项目： | 国家高技术研究发展计划项目(2001AA243052,2003AA243051)，国家自然科学基金项目(30070551)，中华农业科教基金项目(99-03-A-5) |
| 摘 要： | RT-PCR、锚定PCR、cDNA末端快速扩增（RACE）、step out RACE、DDRT-PCR、cDNA代表性差异分析、抑制性削减杂交等都是建立在PCR基础之上的cDNA分子克隆技术。本介绍各种技术的优缺点、应用以及与同类技术进行比较，这些技术现在已经成功应用于大量EST的分离、cDNA库的构建、编码产物未知的差别表达基因的分离以及全长cDNA基因序列的克隆等方面。 |
| 关 键 词： | cDNA代表性差异分析 编码产物 RT-PCR 研究进展 分离 EST 基因序列 克隆技术 RACE DNA分子 |
| 文章编号： | 1009-0002(2004)06-0623-03 |
| 修稿时间： | 2004-04-19 |
Research progress on PCR-based techniques of cDNA gene cloning |
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| CAI Xin,CHEN Hong,,WANG Hong-ying. College of Life Science,Northwest Sci-tech University of Agriculture and Forestry,Yangling , . College of Animal Science and Technology,Northwest Sci-tech University of Agriculture and Forestry,Yanglingi . Research progress on PCR-based techniques of cDNA gene cloning[J]. Letters in Biotechnology, 2004, 15(6): 623-625 | |
| Authors: | CAI Xin CHEN Hong WANG Hong-ying. College of Life Science Northwest Sci-tech University of Agriculture Forestry Yangling . College of Animal Science Technology Northwest Sci-tech University of Agriculture Forestry Yanglingi |
| Affiliation: | CAI Xin1,CHEN Hong2,3,WANG Hong-ying41. College of Life Science,Northwest Sci-tech University of Agriculture and Forestry,Yangling 712100, 2. College of Animal Science and Technology,Northwest Sci-tech University of Agriculture and Forestry,Yanglingi 712100, 3. Institute of Biotechnology,Xuzhou Normal University,Xuzhou 221116, 4. The First Middle School of Zhangxian County,Zhangxian 748300,China |
| Abstract: | RT-PCR, single-sided(anchored) PCR, RACE, step out RACE, DDRT-PCR, cDNA RDA and SSH are all the techniques of cDNA cloning based on PCR. The advantages and disadvantages of each technique were dicussed and compared with those of their counterparts. These techniques are now successfully used in the fields of isolation of numerous ESTs, construction of cDNA libraries, obtaining of differentially expressed new genes and cloning of cDNA in full length. |
| Keywords: | cDNA gene cloning PCR characteristics applications |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |