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Expression of aldehyde dehydrogenase 6 reduces inhibitory effect of furan derivatives on cell growth and ethanol production in Saccharomyces cerevisiae |
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| Authors: | Park Seong-Eon Koo Hyun Min Park Young Kyoung Park Sung Min Park Jae Chan Lee Oh-Kyu Park Yong-Cheol Seo Jin-Ho |
| Affiliation: | a Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea b Emerging Technology Research Center, Samsung Advanced Institute of Technology, Yongin 446-712, South Korea c Bioenergy Research Center, Korea Forest Research Institute, Seoul 130-712, South Korea d Department of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul 136-702, South Korea |
| Abstract: | Yeast dehydrogenases and reductases were overexpressed in Saccharomyces cerevisiae D452-2 to detoxify 2-furaldehyde (furfural) and 5-hydroxymethyl furaldehyde (HMF), two potent toxic chemicals present in acid-hydrolyzed cellulosic biomass, and hence improve cell growth and ethanol production. Among those enzymes, aldehyde dehydrogenase 6 (ALD6) played the dual roles of direct oxidation of furan derivatives and supply of NADPH cofactor to their reduction reactions. Batch fermentation of S. cerevisiae D452-2/pH-ALD6 in the presence of 2 g/L furfural and 0.5 g/L HMF resulted in 20-30% increases in specific growth rate, ethanol concentration and ethanol productivity, compared with those of the wild type strain. It was proposed that overexpression of ALD6 could recover the yeast cell metabolism and hence increase ethanol production from lignocellulosic biomass containing furan-derived inhibitors. |
| Keywords: | Cellulosic ethanol Furan derivatives Saccharomyces cerevisiae Detoxification Aldehyde dehydrogenase 6 |
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