一次给予红藻氨酸对大鼠脑内细胞信息传递通路及癫痫敏感性的作用

本实验给大鼠皮下注射红藻氨酸（ＫＡ，１０ｍｇ／ｋｇ）诱发癫痫活动，７２ｄ后进行深部前梨状皮层（ｄｅｅｐ ｐｒｅｐｙｒｉｆｏｒｍ ｃｏｒｔｅｘ，ＤＰＣ）电点燃刺激（ｋｉｎｄｌｉｎｇ），该组动物点燃形成加速，或再给予同样剂量ＫＡ，其再次诱发的癫痫活动明显加重。ｃ－Ｆｏｓ免疫反应活性（ｃ－Ｆｏｓ－ｉｒ）作为神经元兴奋的标志，标绘再次诱发癫痫活动时大鼠脑内细胞信息传递通路，并与对照组，即７２ｄ前给予生理盐

THE EFFECTS OF SINGLE ADMINISTRATION OF KAINIC ACID ON CELLULAR SIGNAL TRANSMISSION PATHWAY AND SUSCEPTIBILITY TO SEIZURE

In this study, Sprague- Dawley(S. D. ) rats were pretreated with kainic acid (KA,10 mg/kg,i.sc.) 72 days before either deep prepyriform cortex (DPC) kindling or injection of the same dose of KA. The results showed that such pretreatment accelerated the process of kindling, and the KA- induced seizures appeared more severe in behaviours. c-Fos-immunoreactivity (c-Fos-ir) was used to demonstrate the neuronal activity in central nervous system during the provocative dose of KA-induced seizure,as comparing with the control which received normal saline of KA 72 days ago. The results indicated that, the tansmission pathways in two groups were just the same; but the time courses of c-fos and c-jnn expression in KA pretreated group were accelerated asynchronouslys and the synthesis of the proenkephalin mRNA in the Ent was apparently up-regulated. It is suggcsted that, the long-lasting increase in susceptibility to seizure induced by a single administration of KA may be related to the acceleration of the expression of c-fos/jun and the early onset of the expression of proenkephalin mRNA.