人胚DA能神经元移植于帕金森氏病猴模型的TH免疫细胞化学的研究

为了对人胚黑质ＤＡ神经元移植治疗ＰＤ人的临床应用作出客观评估，将８－１２周人胚黑质细胞移植到用ＭＰＴＰ诱发的偏侧ＰＤ猴新纹状体内。实验动物分别存活２个月、５个月和１年后，用ＴＨ免疫细胞化学方法对被移植的人胚ＤＡ细胞的存活和与宿主间的突触联系进行检查。在光镜下可见被移植侧的新纹状体内有ＴＨ阳性细胞，它们成小群散在分布，每小群有３－１０个细胞。ＴＨ阳性细胞的轴突延伸到整个新纹状体，树突呈现出正常发育过

FATE OF HUMAN FETAL DOPAMINE NEURONS TRANSPLANTED INTO RHESUS MONKEY MODEL OF PARKINSON'S DISEASE:A TYROSINE HYDROXYLASE IMMUNOCYTOCHEMICAL STUDY

To predicate the value of human fetal substantia nigra transplantation in clinical treatment of Parkinson's disease (PD), dissociated cells of substantia nigra from 8-12week old abortive human fetus were grafted into the neostriatum of 5 adult rhesus monkeys with hemiparkinsonism induced by unilateral injection of MPTP. At 2, 5 and 12months after transplanting the monkeys were sacrificed for tyrosine hydroxylase (TH)immunocytochemistry to examine the survival and possible synaptic contact of transplanted dopamine (DA) neurons. Transplanted TH immunoreactive cells took a patternof patches scattered in the neostriatum. Each of the cell patches consisted of 3 -1 0 cells. The TH immunoreactive fiber network was seen in the neostriatum. Electron microscopic survey revealed that TH buttons arising from grafted DA neurons formedsymmetric or asymmetric synapses with TH- dendritic shafts/spines, and TH dendrites were seen to form synapses with TH- axons of the host. Additionally, there werea few synapses formed by TH axconal terminals with negative buttons. The results suggest that DA neurons from 8-12 week old abortive human fetus are able to survivegrafting into the neostriatum of monkey, a species phylogenetically very close to human, and to establish reciprocal synaptic connectivity with the host even at 2 monthspost-transplanting. It is, therefore, inferable that embryonic human DA neurons transplanted into human neostriatum may have the same fate as in monkeys.