培养的下丘脑神经细胞电生理特征的衰老性变化

本文采用玻璃微电极细胞内记录技术，研究了培齿０－１４０ｄ新生ＳＤ大鼠下丘脑神经细胞电生理学特征的增龄性变化。以神经突起的生长速度为指标，将细胞体外存活过程分为三期，即恢复期、生长期、衰老期。发现，神经细胞膜时间常数及膜电容于生长期达最大值，而在衰老期显著减小。恢复期膜阻抗最高，生长期显著下降，但衰老期变化不显著。神经细胞可产生单个或多个诱发放电，其静息电位约－３０－－６０ｍＶ，并呈增龄性增高。可见

AGING CHANGES OF THE ELECTRO-PHYSIOLOGICAL CHARACTERISTICS OF HYPOTHALAMIC NEURONS IN CULTURE

The aging changes of the electrophysiological characteristics of the hypothalamic neurons cultured for 0-140 days were studied in neonatal SD rats by the intracellularmicroelectrode recording technique. The growth velocity of the neurites was used as anindex to devide the living process of the neurons into three periods, i. e. recovering period,growth period and aging period. It was found that the time constant and the membrane capacitance reached a top value in the growth period and then decreased significantly in the aging period (P<0. 05, P<0. 01 ). In the recovering period, the membrane resistance reached the highest; and then decreased significantly in growth periodbut did not change significantly in the aging Period. The amplitude of the membraneresting potential was about - 30 - - 60 my and increased with the days in vitro (P<0. 05 ). The neurons could produce single or burst discharges. It appears that certainelectrophysiological characteristics of the hypothalamic neurons in culture could characterize certain aging changes.