Low temperature promotes annexin V expression in newt testis

We examined the effect of low temperatures on annexin V expression in newt testis. When newts were transferred to a low temperature (12 degrees C), up-regulation of annexin V protein was observed in secondary spermatogonia. In primary spermatocytes, high levels of annexin V expression were observed at both 12 degrees C and 22 degrees C, but at 12 degrees C the protein was localized in part of the cytoplasm of primary spermatocytes. These results indicate that in newt testis annexin V is a cold-sensitive protein, suggesting the possibility that annexin V might have a cold stress-related function in newt germ cells.