Novel members of the phosphate regulon in Escherichia coli O157:H7 identified using a whole-genome shotgun approach |
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| Authors: | Yusuke Yoshida Shinichiro SugiyamaTomoya Oyamada Katsushi YokoyamaKozo Makino |
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| Affiliation: | Department of Applied Chemistry, National Defense Academy, 1-10-20 Hashirimizu, Yokosuka 239–8686, Japan |
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| Abstract: | |
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| Keywords: | Apr, ampicillin resistance ATP, adenosine triphosphate β-gal, β-galactosidase DIG, digoxigenin DTT, dithiothreitol DNase I, deoxyribonuclease I EDTA, ethylenediaminetetraacetic acid EMSA, electrophoretic mobility shift assay LB, Luria&ndash Bertani (medium) ORF, open reading frame PCR, polymerase chain reaction pho box, a conserved sequence for PhoB binding pho regulon, phosphate regulon Pi, phosphate psi, phosphate-starvation-inducible SDS, sodium dodecyl sulfate TE-saturated phenol, Tris&ndash EDTA-saturated phenol TGHP, Tris&ndash glucose medium with high phosphate TGLP, Tris&ndash glucose medium with low phosphate UV, ultraviolet X-gal, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside |
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