Novel members of the phosphate regulon in <em>Escherichia coli</em> O157:H7 identified using a whole-genome shotgun approach期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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*Novel members of the phosphate regulon in Escherichia coli* O157:H7 identified using a whole-genome shotgun approach**

Authors:	Yusuke Yoshida Shinichiro SugiyamaTomoya Oyamada Katsushi YokoyamaKozo Makino

Institution:	Department of Applied Chemistry, National Defense Academy, 1-10-20 Hashirimizu, Yokosuka 239–8686, Japan

Abstract:

Keywords:	Ap^r ampicillin resistance ATP adenosine triphosphate β-gal β-galactosidase DIG digoxigenin DTT dithiothreitol DNase I deoxyribonuclease I EDTA ethylenediaminetetraacetic acid EMSA electrophoretic mobility shift assay LB Luria&ndash Bertani (medium) ORF open reading frame PCR polymerase chain reaction pho box a conserved sequence for PhoB binding pho regulon phosphate regulon Pi phosphate psi phosphate-starvation-inducible SDS sodium dodecyl sulfate TE-saturated phenol Tris&ndash EDTA-saturated phenol TGHP Tris&ndash glucose medium with high phosphate TGLP Tris&ndash glucose medium with low phosphate UV ultraviolet X-gal 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside
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