Association of three polymorphisms of scavenger receptor class BI gene (exon8, exon1, intron5) with coronary stenosis in a coronary Tunisian population |
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Authors: | Jihène Rejeb Asma Omezzine Imen Boumaiza Lamia Rebhi Slim Kacem Nabila Ben Rejeb Naoufel Nabli Ahmed Ben Abdelaziz Essia Boughzala Ali Bouslama |
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Institution: | 1. Biochemistry Department, UR MSP 28/04, Sahloul University Hospital, Sousse, Tunisia;2. Cardiology Department, Sahloul University Hospital, Sousse, Tunisia;3. Information System Direction, Sahloul University Hospital, Sousse, Tunisia |
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Abstract: | BackgroundThe potential role of scavenger receptor class BI (gene name SCARB1) in the regulation of lipoproteins metabolism and atherosclerosis has attracted considerable interest. We tested the relationship of SCARB1 polymorphisms with significant coronary stenosis (SCS) and lipid profile in a coronary Tunisian population.MethodsThree SCARB1 polymorphisms (exon8 (C/T), exon1 (G/A), intron5 (C/T)) were studied in 316 Tunisian patients undergoing coronary angiography. SCS was defined as a luminal narrowing of ≥ 50% in at least one major coronary artery. Lipid profile was measured. Genotyping was performed using PCR–RFLP.ResultsIndividuals with TT genotypes of exon8 were associated with higher concentrations of plasma HDL-C and ApoAI in the group without SCS. Carriers of T allele of exon8 were associated with 41% lower risk of SCS. This protective effect seemed to be particularly significant in women, nondiabetics and nonsmokers. Subjects homozygous for the variant allele of intron5 were significantly associated with an increased risk of SCS, particularly in smokers. AA genotype of exon1 was associated with an increased risk of SCS in diabetics and in patients with metabolic syndrome. The (CAT) haplotype was associated with increase in the risk of SCS compared to the wild haplotype and had a 4-fold greater risk of SCS than patients with haplotype (TGC) which seems to be the most protective against SCS.ConclusionCarriers of T allele of exon8 in SCARB1 seemed to increase HDL-C and ApoAI concentrations and reduce the risk of SCS. The intron5, exon1 and (CAT) haplotype seemed to have an atherogenic effect. |
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Keywords: | CAD coronary artery disease CHD coronary heart disease CIs confidence intervals HDL high-density lipoprotein HDL-C HDL-cholesterol HTA hypertension LD linkage disequilibrium LDL-C low density lipoprotein cholesterol MS metabolic syndrome ORs odds ratios PCR polymerase chain reaction RCT reverse cholesterol transport SCS significant coronary stenosis SNPs single nucleotide polymorphisms SCARB1 gene name of SR-BI SR-BI scavenger receptor class B type I TC total cholesterol TG triglyceride |
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