| 14个染色体区带特异性探针池的构建 |
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| 引用本文： | 夏家辉,杨毅,戴和平,潘乾,李麓芸.14个染色体区带特异性探针池的构建[J].遗传学报,1994(4). |
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| 作者姓名： | 夏家辉 杨毅 戴和平 潘乾 李麓芸 |
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| 作者单位： | 湖南医科大学医学遗传学国家重点实验室 |
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| 摘 要： | 本文运用人类染色体显微切割和ＰＣＲ技术，成功地构建了１４个染色体区带特异性探针池，并通过染色体原位杂交证明它们均分别来源于相应的被切割的染色体区带。
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| 关 键 词： | 探针池，染色体区带，显微切割，PCR，荧光原位杂交 |
Construction of 14 DNA Probe Pools for Specific Chromosomal Bands |
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| Xia Jiahui, Yang Yi, Dai Heping, Pan Qian, Li Luvun.Construction of 14 DNA Probe Pools for Specific Chromosomal Bands[J].Journal of Genetics and Genomics,1994(4). |
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| Authors: | Xia Jiahui Yang Yi Dai Heping Pan Qian Li Luvun |
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| Institution: | State Key Laboratory of Medical Genetics of China Changsha Hunan 410078 |
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| Abstract: | Using the technique of human chromosome microdissection and PCR. we have successfully constructed 14 DNA probe pools for specific chromosomal bands. The chromosome in situ suppression (CISS) hybridization proved that DNA probe pools were derived from the chromosomal bands 1q32, 2q33, 5q13, 6p21, 8q24.1,11q13, 13q12,15q11.2-12,1p36.3,1q42,11q23-qter,17pl3.2-pter,17q11.2 and 21q21.2-q22.1 respectively. |
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| Keywords: | Probe pool Chromosomal banding Microdissection PCR CISS hybridization |
| 本文献已被 CNKI 等数据库收录！ |
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