卵巢癌肝转移灶高表达基因SFT2D1的生物信息学分析

目的:研究肿瘤原发灶和转移灶的基因表达差异,并采用生物信息学方法对一条卵巢癌肝转移灶高表达基因SFT2D1进行初步分析。方法:分别将卵巢癌原发灶和肝转移灶组织标本mRNA用Cy3-dUTP和Cy5-dUTP标记后与表达谱芯片杂交,通过信号扫描、处理后获得两者的表达差异基因。并用生物信息学方法对一条无功能研究的新基因SFT2D1进行初步分析,阐明了它的基因结构、染色体定位、编码蛋白质的理化性质、亚细胞定位、蛋白质功能域等信息。并对多物种中的相似性蛋白进行了系统进化分析。结果:表达谱芯片发现了共272条差异表达基因。对新基因SFT2D1的上述性质进行了有效的预测,基本明确了该基因编码蛋白为一内质网跨膜蛋白,可能参与肿瘤转移相关蛋白的合成与加工。结论:表达谱芯片技术是一种研究肿瘤转移基因表达差异的有效的高通量研究方法。通过生物信息学分析,表明新基因SFT2D1是一个有肿瘤转移研究价值的新靶点。

Bioinformatics analysis of SFT2D1--a highly expressed gene in hepatic metastasis of ovarian cancer

Objective: To explore the differently expressed genes between primary lesion(PL) and metastasis of tumor,and perform an initial bioinformatics analysis on a novel gene,SFT2D1,which is highly expressed in hepatic metastasis(HM).Methods: The mRNA of the tissues in PL and HM were respectively marked with Cy3-dUTP and Cy5-dUTP and hybridized with expression profile microarray,and the differently expressed genes were obtained.Initial bioinformatics analysis was performed on a novel gene named SFT2D1 without functional researches at present.Its gene structure,genome localization,the physical and chemical characteristics of the coded protein,subcellular localization,functional domain and so on were clarified,and the systematic evolution analysis was performed on the similar proteins of several species.Results: Using expression profile microarray,272 differently expressed genes were uncovered.Through efficient bioinformatics analysis,SFT2D1 protein was fundamentally identified to be a trans-membrane protein located on endoplasmic reticulum,and may involve in the synthesis and processing of metastasis related proteins.Conclusion: Microarray is an efficient and high-throughout strategy for the detection of differently expressed genes.And SFT2D1 has been thought to be a potential target for tumor metastasis researches by bioinformatics analysis.