Analysis of polyamine metabolism in soybean seedlings using 15N-labelled putrescine |
| |
Authors: | Ohe Masato Kobayashi Masaki Niitsu Masaru Bagni Nello Matsuzaki Shigeru |
| |
Affiliation: | Department of Biochemistry, Dokkyo University School of Medicine, Mibu, Tochigi 321 0293, Japan. masatooe@dokkyomed.ac.jp |
| |
Abstract: | The translocation and metabolism of polyamines during soybean germination were studied using 15N-labelled putrescine as a precursor. Both 15N-labelled and unlabelled polyamines were simultaneously detected using a novel application of ionspray ionization-mass spectrometry. 15N-putrescine was rapidly transported to the shoots and roots, where it was converted to spermidine and spermine. The main 15N-polyamine that accumulated in the root was 15N-spermine. It was found that there were differences in the way endogenous putrescine and exogenous 15N-putrescine were metabolized in soybean seedlings. |
| |
Keywords: | Glycine max Leguminosae Soybean Metabolism Translocation Polyamine |
本文献已被 ScienceDirect PubMed 等数据库收录! |