用吸胀初期超微弱化学发光的方法快速检测水稻种子活力

用高灵敏度的单光子计数系统探测了不同贮藏年份的水稻 (OryzasativaL .)品种 80 72 2吸胀初期的超微弱化学发光 (ultraweakchemilumines cence,UCL)。观测到水稻种子吸胀初期 ( 0～ 30min)UCL强度与其老化程度呈负相关 ,水稻种子贮藏时间越长 ,萌发率越低 ,UCL的强度越弱 ,水稻种子UCL强度与萌发率呈极显著正相关。用灵敏的能与单线态氧 ( 1 O2 )反应产生化学发光的发光试剂MCLA ( 2 methyl 6 ( p methoxyphenyl) 3,7 dihy droimidazo[1 ,2 a]pyrazin 3 one) ,检测到吸胀初期水稻种子有单线态氧 ( 1 O2 )的生成 ,产生1 O2 的量与其萌发率呈高度正相关。水稻种子吸胀初期单线态氧的生成是超微弱化学发光的重要诱发因素之一。种子吸胀初期UCL的差异有望成为一种快速、定量、无损伤检测水稻种子活力的新方法。

Rapid Determination of Rice Seed Vigor by Ultraweak Chemiluminescence During Early Imbibition

With high-sensitivity single-photon counter, ultraweak chemiluminescence (UCL) study on rice (Oryza sativa L.) seeds stored for different years was carried out. The intensity of UCL was observed to be related to the degree of aging of rice seeds during early imbibition (0-30 min). Rice seeds with longer storage time had lower intensity of UCL during early imbibition. The germination rate of rice seeds showed a significant positive correlation with the intensity of UCL. Singlet oxygen (1O 2) in rice seeds during early imbibition was investigated by chemiluminescence (CL) method using a sensitive CL probe MCLA (2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo pyrazin-3-one). The output of 1O 2 showed a high positive correlation with the germination rate. Analysis based on the experimental results demonstrated that UCL in rice seeds during early imbibition could be attributed mainly to singlet oxygen (1O 2). The UCL technique is a potential way in elaborating a fast, quantitative and non-invasive method for rapid determination of the degree of aging rice seed.