Inhibitor of 2',5'-oligoadenylate synthetase induced in human T lymphoblastoid cell line treated with deoxyadenosine, deoxycoformycin and interferon |
| |
Authors: | T Heike K Katamura M Kubota K Shinomiya H Mikawa |
| |
Affiliation: | Department of Pediatrics, Faculty of Medicine, University of Kyoto, Japan |
| |
Abstract: | The effect of deoxyadenosine (dAdo) with deoxycoformycin on the induction of 2',5'-oligoadenylate synthetase by interferon was investigated. After semi-purification through poly(I):poly(C) gel, the activity was similar in control and dAdo-treated cells. However, the activity in the crude extract decreased with rising concentrations of dAdo. On the other hand, the level of 2'-phosphodiesterase, which is also induced by interferon and degrades 2',5'-oligoadenylate, showed no significant change after dAdo treatment. Thus, the crude extract was speculated to contain an inhibitor of 2',5'-oligoadenylate synthetase. Further characterization of the inhibitor revealed that inhibition was not due to dATP accumulation in cells. |
| |
Keywords: | interferon IFN 2′,5′-oligoadenylate 2-5A severe combined immunodeficiency disease SCID adenosine deaminase ADA deoxycoformycin dCF deoxyadenosine dAdo high performance liquid chromatography HPLC |
本文献已被 ScienceDirect 等数据库收录! |
|