Interaction of methyl green with an oligonucleotide in intramolecular duplex and triplex conformations |
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Authors: | Maurice Durand Claire Gondeau J C Maurizot |
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Institution: | (1) Centre de Biophysique Moléculaire, Université Orléans, Rue Charles Sadron, F-45071 Orléans Cedex 2, France, FR |
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Abstract: | Interaction of methyl green with the oligonucleotide 5′-dGGAAAAGG-T4]-GGAAAAGG-T4]-CCTTTTCC (where T4] is a nucleotide sequence of four thymines) in hairpin duplex and in intramolecular triplex structures has been studied by
circular dichroism. We found that methyl green binding to the duplex form shows a complex pattern, exhibiting an exciton contribution
when the number of bound molecules increases. Differences between this pattern and previously published results on other DNAs
reveals the presence of different types of complexes. In contrast to previous findings with the triple helix poly(dA).2poly(dT)
we show that the methyl green is not totally excluded from this triplex structure made of Pur:Pur:Pyr triplets.
Received: 15 July 1997 / Accepted: 22 September 1997 |
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Keywords: | Methyl green DNA triplex Pur:Pur:Pyr triplet Circular dichroism |
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