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Genetic mapping of the polycystic kidney gene,pcy, on mouse chromosome 9 |
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| Authors: | Shizuko Nagao Tomomasa Watanabe Noboru Ogiso Tohru Marunouchi Hisahide Takahashi |
| Institution: | 1. Laboratory Animal Center, Institute for Comprehensive Medical Science, Fujita Health University, 470-11, Aichi, Japan 2. Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, 060, Sapporo, Japan 3. Division of Cell Biology, Institute for Comprehensive Medical Science, Fujita Health University, 470-11, Aichi, Japan |
| Abstract: | The murine polycystic kidney disease gene,pcy, is an autosomal recessive trait located on chromosome 9. To determine the genetic locus ofpcy, 222 intraspecific backcross mice were obtained by mating C57BL/6FG-pcy andMus molossinus. Restriction fragment length polymorphism analysis of 70 of the 222 backcross progeny showed thatpcy, dilute coat color (d), and cholecystokinin (Cck) were located in the orderd—pcy—Cck from the centromere. Simple sequence repeat length polymorphism analysis of DNA of all 222 backcross mice was carried out using four markers which were located near the central regions ofd andCck. One and eight recombinations were detected betweenD9Mit24 andpcy and betweenD9Mit16 andpcy, respectively. However, no recombinant was observed amongpcy, D9Mit14, andD9Mit148. These findings strongly suggest thatD9Mit14 andD9Mit148 are located near thepcy gene and are good markers for chromosomal walking to this gene. |
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