Loss of heterozygosity is induced in <Emphasis Type="Italic">Candida albicans</Emphasis> by ultraviolet irradiation |
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| Authors: | Yukinobu Takagi Rinji Akada Hidehiko Kumagai Kenji Yamamoto Hisanori Tamaki |
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| Institution: | (1) Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku Kyoto, 606-8502, Japan;(2) Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai Ube, 755-8611, Japan;(3) Research Institute of Agricultural Resources, Ishikawa Prefectural University, Nonoichi-cho Ishikawa, 920-8580, Japan;(4) Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Korimoto Kagoshima, 890-0065, Japan |
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| Abstract: | Candida albicans is a human fungal pathogen and has been extensively studied because of its clinical importance. Comprehensive gene analyses
have, however, made little progress. This is because of the diploid and asexual characteristics of the fungus that hamper
gene disruptions. In this study, we found that ultraviolet (UV) irradiation, as well as mutagen treatment, strongly stimulated
loss of heterozygosity (LOH) in strains harboring artificially constructed heterozygosity. UV-induced LOH occurred more frequently
in cells within the logarithmic phase of growth compared to those within the stationary phase of growth. This was observed
at all loci tested on chromosome 7, except for a locus neighboring the centromere. C. albicans RAD52, whose orthologue in Saccharomyces cerevisiae was reported to be involved in DNA repair by homologous recombination, was shown to be required for UV-induced LOH. These
results suggest that high efficiency LOH caused by UV irradiation could be a prominent tool for gene analyses in C. albicans. |
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| Keywords: | Candida albicans Loss of heterozygosity UV RAD52 |
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