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| 微波辅助提取灰树花多糖工艺研究 | |
| 引用本文： | 唐小俊,池建伟,何焕清,魏振承,张雁,施英. 微波辅助提取灰树花多糖工艺研究[J]. 天然产物研究与开发, 2006, 18(5): 841-845 |
| 作者姓名： | 唐小俊 池建伟 何焕清 魏振承 张雁 施英 |
| 作者单位： | 1. 广东省农业科学院农业生物技术研究所,农业部功能食品重点开放实验室,广州,510610 2. 广东省农业科学院蔬菜研究所,广州,510640 3. 广东省农业科学院蚕业与农产品加工研究所,广州,510610 |
| 基金项目： | 广东省农业攻关项目(2004B20101004) |
| 摘 要： | 采用提取时间、微波功率、液料比的单因素试验和正交试验法优化微波辅助提取灰树花多糖条件.结果表明,以净多糖得率为指标,影响微波辅助提取灰树花多糖的主次因素为:提取时间＞微波功率＞液料比,并且提取时间和微波功率的影响达到了极显著水平.灰树花多糖最佳提取工艺条件为:提取时间为10 min,微波功率为80%(全功率为800 W),液料比为25∶1.创立了一种用苯酚-硫酸法测定多糖时排除蛋白质干扰的方法. |
| 关 键 词： | 灰树花 多糖 微波 提取 蛋白质干扰 |
| 文章编号： | 1001-6880(2006)05-0841-05 |
| 修稿时间： | 2005-09-29 |
Microwave-assisted Extraction Technology of Polysaccharides from Grifla frondosa |
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| TANG Xiao-jun,CHI Jian-wei,HE Huan-qing,WEI Zhen-cheng,ZHANG Yan,SHI Ying. Microwave-assisted Extraction Technology of Polysaccharides from Grifla frondosa[J]. Natural Product Research and Development, 2006, 18(5): 841-845 | |
| Authors: | TANG Xiao-jun CHI Jian-wei HE Huan-qing WEI Zhen-cheng ZHANG Yan SHI Ying |
| Abstract: | The conditions of polysaccharides extraction from Grifola frondosa including extraction time,microwave power and water/dry weight ratio were studied by single factor experiment and orthogonal design guided by the pure polysaccharides extraction rate.The results show that the influencing level of every factors during extracting polysaccharides from Grifola frondosa is as follows:extraction time > microwave power > water/dry weight ratio,and the influnces of extraction time and microwave power were very notable.The optimum extraction conditions were as follows:10 min,80% microwave power(full power is 800 W),25:1(water/dry weight).A method was set up to remove the disturbances of protein on phenol-sulphate acid method during measuring of polysaccharides. |
| Keywords: | Grifola frondosa polysaccharides microwave extraction disturbance of protein |
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