Differentiation of Legionella species by soluble protein patterns in polyacrylamide slab gels |
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| Authors: | D A Ferguson W R Mayberry |
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| Affiliation: | Department of Microbiology, Quillen-Dishner College of Medicine, East Tennessee State University, Johnson City 37614. |
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| Abstract: | Water-soluble protein extracts were obtained from representative strains of eleven species of Legionella and examined by polyacrylamide slab gel electrophoresis (PAGE). The eleven species included: L. pneumophila (serogroups 1-9), L. micdadei, L. longbeachae (serogroups 1 and 2), L. gormanii, L. dumoffii, L. bozemanii, L. jordanis, L. oakridgensis, L. wadsworthii, L. feeleii and L. maceachernii. Species identification was verified by gas-liquid chromatography of the non-hydroxy, monohydroxy and dihydroxy fatty acids. Strains within each species shared common patterns of protein banding. Each species had a distinctive protein banding pattern. Results indicate that PAGE of water soluble protein extracts is a useful technique for identification of Legionella isolates. |
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