UPTAKE OF CALCIUM BY SUBCELLULAR FRACTIONS ISOLATED FROM OUABAIN-TREATED CEREBRAL TISSUES |
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Authors: | W L Stahl P D Swanson |
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Institution: | Division of Neurology, Department of Medicine and Department of Physiology and Biophysics, University of Washington School of Medicine and the Neurochemistry Laboratory, Veterans Administration Hospital, Seattle, Washington |
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Abstract: | Abstract— Slices of cerebral cortex were incubated in medium containing 0·75 or 2·8 mM 45CaCl2, in the presence or absence of 0·01–0·1 m m -ouabain. Ouabain induced accumulation of calcium by slices to a maximum of 4 μmoles/g of tissue/hr (0·75 m m -CaCl2 in the medium) and to 8 μmoles/g of tissue/hr (2·8 m m -CaCl2 in the medium). Accumulation of Ca2+ occurred more slowly than loss of K+ from the slices and more closely resembled the pattern of Na+ uptake. Mitochondrial fractions isolated from ouabain-treated slices contained significantly more calcium than controls. Inclusion of EDTA in the homogenization medium resulted in decreased amounts of particulate-bound calcium. The effect of ouabain on accumulation of calcium is discussed with regard to possible relationships to processes of active and passive transport. |
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