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Rapid procedure for preparation of macrophage plasma membrane
Authors:Y Shibata  Y Abiko  H Arii  M Sone  H Takiguchi
Affiliation:1. Marian Smoluchowski Institute of Physics, Jagiellonian University, ul. Łojasiewicza 11, Kraków 30-348, Poland;2. GREMI, Université d’Orléans/CNRS, 63 avenue de Lattre de Tassigny, Bourges cedex F-18020, France;3. Institute of Physics, University of Opole, ul. Oleska 48, Opole 45-052, Poland;1. Research Fellow of Japan Society for the Promotion of Science (JSPS), Japan;2. Environmental and Renewable Energy Systems Division, Graduate School of Engineering, Gifu University, 1-1 Yanagido, Gifu-shi, Gifu 501-1193, Japan;3. Department of Informatics, Faculty of Industrial Technology, Universitas Atma Jaya Yogyakarta, Jl. Babarsari 44, Yogyakarta 55281, Indonesia;4. Research Group of Sustainable Thermofluids, Universitas Sebelas Maret, Jl. Ir. Sutami 36A Kentingan, Surakarta 57126, Indonesia;5. Department of Mechanical Engineering, Faculty of Engineering, Universitas Sebelas Maret, Jl. Ir. Sutami 36A Kentingan, Surakarta 57126, Indonesia;1. Department of Chemical Engineering, Myongji University, Yongin 17058, Republic of Korea;2. Clean Energy Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea
Abstract:This report describes a simple and efficient procedure for the isolation of plasma membrane from guinea pig peritoneal macrophages. The use of polycationic beads (Affi-gel 731 beads) facilitates rapid and high-clear separation of plasma membrane within 30 min. The final plasma membrane coated beads fraction has high specific activities of marker enzymes with little contamination with mitochondrial, lysosomal or cytoplasmal markers.
Keywords:
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