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In vitro migration of Dictyocaulus viviparus larvae: migration of the infective stage inagar gel.
Authors:R J Jorgensen
Institution:The Danish Agricultural and Veterinary Research Council, Parasitological Research Group, Institute of Internal Medicine, Royal Veterinary and Agricultural University, Bülowsvej 13, 1870 Copenhagen V, Denmark
Abstract:The infective stage of the cattle lungworm, Dictyocaulus viviparus, was able to migrate in agar gel when activated by bile. The number of larvae which penetrated the upper surface of a 2 mm thick agar layer was counted and found to be independent from the agar concentration. Larvae which had migrated out of the agar remained on the surface and did not re-enter. The agar migration process was temperature dependent. Influence of time as well as dependency of agar thickness was investigated. The significance of these findings is discussed.
Keywords:migration  nematode  agar gel  bile
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