Oligonucleotide microarray for the subtyping of influenza virus a neuraminidase |
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| Authors: | V. A. Ryabinin E. V. Kostina A. A. Neverov G. A. Maksakova A. N. Sinyakov |
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| Affiliation: | 1.Institute of Chemical Biology and Fundamental Medicine, Siberian Branch,Russian Academy of Sciences,Novosibirsk,Russia;2.Laboratory of Method Development, Center for Biologics Evaluation and Research,U.S. Food and Drug Administration,Rockville,USA |
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| Abstract: | A microarray for the subtyping of influenza A neuraminidase is presented. The selection of oligoprobes proceeded in two steps. The first step included the selection of peptides specific for each subtype of neuraminidase. At the second step, the oligoprobes were calculated using the found peptide structures with the subsequent additional selection of the most specific and representative probes. From 19 to 24 probes were used for the determination of each neuraminidase subtype. The microarray testing for 19 samples with the most widespread types (N1 and N2) specifies in an unequivocal definition 18 of them and only 1 isolate has not been identified. |
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