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犬科的线粒体细胞色素b DNA序列及其分子系统学研究
引用本文:陈永久,张亚平,邹希明. 犬科的线粒体细胞色素b DNA序列及其分子系统学研究[J]. 遗传学报, 2000, 27(1): 7-11
作者姓名:陈永久  张亚平  邹希明
作者单位:中国科学昆明动物研究所细胞与分子进化开放研究实验室,昆明
基金项目:国家自然科学基金,云南省科委“二百人计划”资助
摘    要:通过对犬科的赤狐、蓝狐、貉和狼4种的线粒体细胞色素b约372bpDNA片段序列分析,结合GenBank中狗、西门豺和非洲野犬3种的该区段DNA序列的比较,共发现113个核苷酸位点存在变异(约30%)。NJ法构建的分子系统树显示,非洲野犬最先从犬科动物中分化出来;犬属的我狼、狗和西门豺等3种为系统树上独立的一支,且其分歧的时间较赤狐、蓝狐和貉早;赤狐和蓝狐具有较近的亲缘关系。上述结果与形态的观点基本

关 键 词:犬科 线粒体DNA 细胞色素b 分子系统学

Molecular Phylogeny of Canidae Using Mitochondrial Cytochrome b DNA Sequences
Chen Yong-Jiu,Zhang Ya-Ping,Zuo Xi-Ming. Molecular Phylogeny of Canidae Using Mitochondrial Cytochrome b DNA Sequences[J]. Journal of Genetics and Genomics, 2000, 27(1): 7-11
Authors:Chen Yong-Jiu  Zhang Ya-Ping  Zuo Xi-Ming
Abstract:372hp mitochondrial cytochrome b DNA of blue fox, red fox, raccoon dog, and wolf were sequenced. Combined with the DNA fragments of dog, simien fox, and African wild dog, sequences were aligned and analyzed. There are 113 nucleotide sites substituted (30%).Molecular phylogenetic tree constructed by NJ method suggests that African wild dog is the earliest divergent. Wolf, dog, and simien fox which belong to genus Canis are combined into one branch which diverged earlier than raccoon dog, red fox and blue fox. Red fox is more related to blue fox than the other animals. The above result is in consistent with that of morphology.
Keywords:Canidae  mitochondrial DNA  cytochrome b  molecular phylogeny
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