基因枪法转基因水稻中hpt基因稳定遗传

基因枪转化将潮霉素磷酸转移酶基因（ｈｐｔ）导入粳稻品种７７１７０，获得可育的转基因植株，研究外源基因遗传的稳定性。自交后代（Ｔ１和Ｔ２）经潮霉素筛选获得抗性植株和敏感植株，分子鉴定结果表明抗性植株带有ｈｐｔ基因，而敏感植株中没有ｈｐｔ基因存在。Ｔ１和Ｔ２代中潮霉素抗性表现为显性单基因位点的遗传方式，符合孟德尔分离规律，并得到分子鉴定结果的证实。Ｓｏｕｔｈｅｒｎ杂交结果显示，ｈｐｔ基因多拷贝整合在水

Stable Inheritance of hpt Gene in Transgenic Rice Plants Mediated by Biolistic Bombardment

Japonica rice cv 77170 was transformed with PBI222 carrying hygromycin phosphotransferase gene (hpt) by using biolistic bombardment and fertile transgenic rice plants were obtained. In TI and most T2 generation the Performance of hygromycin resistance was normal. Only in T2 progeny of SG-15 some lines showed decreased resistance. Mendelian inheritance of hygromycin resistance was showed as single dominant locus and proved by Southern blotting analysis in T1 and T2 generation of all transgenic plants. Multiple copies of hpt integrated into rice genome. These copies linked closely and inherited stablly. Msp I digested Southern blotting showed that methylation of lip t existed commonly in transgenic plants and their progeny, therefore uncomplete silencing of hp t in some T2 progeny of SG-15 was not associated with hpt methylation probably.