| 小麦及其近缘物种中小分子量核糖核酸酶的初步分析 |
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| 引用本文： | 赵慧,刘坤凡,王道文.小麦及其近缘物种中小分子量核糖核酸酶的初步分析[J].遗传学报,2000,27(5):423-427. |
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| 作者姓名： | 赵慧 刘坤凡 王道文 |
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| 作者单位： | 中国科学院遗传研究所植物细胞与染色体工程国家重点实验室,北京,100101 |
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| 基金项目： | 中国科学院重大项目特支经费资助!（K295T－02－02－17） |
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| 摘 要： | 应用ＲＮａｓｅ功能胶体系，在小麦及其相关物物中发现１组小分子量核糖核酸酶（ＲＮａｓｅ）。ＲＮａｓｅ的分子量变化范围在６．５～１４ｋＤａ间，低于已报道的大多数植物ＲＮａｓｅ的分子量。小分子量ＲＮａｓｅ在幼苗中大量表达，并且在降解ＲＮＡ底物时，随着缓冲液ＰＨ值及离子浓度的不同而有所变化。在休眠及发芽泪科种 现几种可能不同的小分子量ＲＮａｓｅ。在发芽过程中，其中２种ＲＮａｓｅ的少变化不明显，而另外２种Ｒ
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| 关 键 词： | 小麦 近缘种 RNase功能胶 核糖核酸酶 小分子量 |
A Preliminary Analysis on a Group of Low Molecular Weight RNases in Wheat and Related Species |
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| ZHAO Hui,LIU Kun-Fan,WANG DaoF-Wen.A Preliminary Analysis on a Group of Low Molecular Weight RNases in Wheat and Related Species[J].Journal of Genetics and Genomics,2000,27(5):423-427. |
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| Authors: | ZHAO Hui LIU Kun-Fan WANG DaoF-Wen |
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| Abstract: | By employing RNase activity gel analysis, we detected a novel group of RNases in wheat and related species. The molecular weight of the RNases varied from 8-14 kDa, which was lower than that of most plant RNases characterized previously. The small RNases expressed abundantly in seedlings and differed in their optimal pH and ionic requirement in digesting RNA substrate. Several members of the small RNases were detected in dormant and germinating wheat seeds. During germination, the activity of two RNases did not change significantly whereas that of the other two RNases showed a gradual pattern of decrease and increase, respectively. |
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| Keywords: | RNase wheat germinating seeds RNase activity gel |
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