Characterization of Geranium (Pelargonium graveolens) Chloroplast EF-Tu cDNA |
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Authors: | Chul Joo Kang Min Goo Lee Young Sil Cho Joong Won Lee Yoon Jung Kyung Jeong Sheop Shin Eun-Soo Kim Jeong-Kook Kim |
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Affiliation: | (1) Department of Biology, Korea University, Seoul, 136-701, Korea;(2) Graduate School of Biotechnology, Korea University, Seoul, 136-701, Korea;(3) Department of Biology, Konkuk University, Seoul, 136-701, Korea |
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Abstract: | A geranium (Pelargonium graveolens) chloroplast translational elongation factor EF-Tu (tufA) cDNA was isolated. The geranium tufA cDNA is 1,584 bp long with 20 bp of 5 untranslated region (UTR) and 139 bp of 3 UTR. It encodes 474 amino acids including a putative chloroplast transit peptide of 65 amino acids. The deduced polypeptides of the geranium tufA cDNA contains four GTP binding sequences in its N-terminal region and two chloroplast EF-Tu signature regions in the C-terminal region. The predicted molecular weight of the mature geranium chloroplast EF-Tu protein was about 45,000 and its amino acid sequence identity with the chloroplast EF-Tu proteins of tobacco, pea, Arabidopsis, rice, and soybean ranges from 85% to 91%. The geranium tufA appears to exist as a single copy gene like Arabidopsis and rice, whereas other known dicot plants have more than one copy in their nuclear genomes. |
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Keywords: | Chloroplast Elongation Factor EF-Tu Geranium |
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