| 用A蛋白夹层酶联免疫吸附法及病毒与葡萄球菌共凝集法检测黑曲霉病毒 |
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| 引用本文： | 刘宏迪,梁平彦.用A蛋白夹层酶联免疫吸附法及病毒与葡萄球菌共凝集法检测黑曲霉病毒[J].病毒学报,1988(2). |
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| 作者姓名： | 刘宏迪 梁平彦 |
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| 作者单位： | 中国科学院微生物研究所 北京
(刘宏迪)，中国科学院微生物研究所 北京(梁平彦) |
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| 摘 要： | 用葡萄球菌A蛋白夹层酶联免疫吸附(PAS-ELISA)和病毒-葡萄球菌共凝集试验(SA-test),检测了葡萄糖淀粉酶生产菌中的黑曲霉病毒(AsPergillus niger virus)含量。证实了酶产量不同的黑曲霉变异株中的病毒含量与酶产量高低有相关性。并讨论了用上述技术检测病毒量作为快速简便方法筛选高酶产量菌种的可能性。
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| 关 键 词： | A蛋白夹层酶联免疫吸附法 病毒菌体共凝集试验 黑曲霉病毒 葡萄糖淀粉酶 |
DETECTION OF MYCOVIRUS BY STAPHYLOCOCCUS PROTEIN A SANDWICH ELISA AND VIRUS-STAPHYLOCOCCAL CO-AGGLUTINATION TEST |
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| Liu Hongdi Liang Pingyan.DETECTION OF MYCOVIRUS BY STAPHYLOCOCCUS PROTEIN A SANDWICH ELISA AND VIRUS-STAPHYLOCOCCAL CO-AGGLUTINATION TEST[J].Chinese Journal of Virology,1988(2). |
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| Authors: | Liu Hongdi Liang Pingyan |
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| Abstract: | Virus from Aspergillus niger, a glucoamylase producer, was detected by protein A sandwich-ELISA (PAS-ELISA) and virus-staphylococcal co-gglua-tination test (SA test). The vrus titres in virus infected A. niger strains with different Ievel of glucoamylase product were described and the possibi-lities of usiag these technique as rapid, simple raethods in the seletion of high yield enzyme producers were discussed. |
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| Keywords: | Protin A sandwich ELISA Virus Staphylococcal co-aggluti-uation test Aspergillus niger virus Glucoamylase |
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