An elicitor-induced cDNA from aerial yam (Dioscorea bulbifera L.) encodes a pathogenesis-related type 4 protein |
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| Authors: | R Rompf G Kahl |
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| Institution: | (1) Plant Molecular Biology, Biocentre, Johann Wolfgang Goethe-University, Marie-Curie-Strasse 9, D-60439 Frankfurt/Main, Germany Fax: +49-69-79829268 e-mail: kahl@em.uni-frankfurt.de, DE |
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| Abstract: | We established Dioscorea bulbifera (aerial yam) cell suspension cultures to study the expression of defense-related genes upon elicitation with the yam pathogenic
ascomycete Colletotrichum gloeosporioides. The induction of phenylalanine-ammonia lyase (PAL) mRNA, coding for a key enzyme involved in phytoalexin biosynthesis, was
observed upon elicitation. Using RT-PCR, we isolated an elicited cDNA clone with an open reading frame of 453 nucleotides
which showed high homology to cDNA sequences of pathogenesis-related proteins belonging to the PRP-4 group. Yam PRP-4 expression is increased in elicited cell cultures as well as in elicited leaves, and is encoded by a small multigene family.
This is the first example for the cloning of a cDNA that might be involved in defense reactions of yam plants.
Received: December 1997 / Revision received: June 1998 / Accepted: November 1998 |
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| Keywords: | Dioscorea bulbifera Cell suspension cultures Elicitor-responsive genes PRP-4 |
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