METHODS OF ASSESSING THE SPORICIDAL EFFICIENCY OF AN ULTRA-HIGH-TEMPERATURE MILK STERILIZING PLANT I. EXPERIMENTS WITH SUSPENSIONS OF SPORES IN WATER |
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| Authors: | D J WILLIAMS J G FRANKLIN HELEN R CHAPMAN L F L CLEGG |
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| Institution: | National Institute for Research in Dairying, University of Reading |
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| Abstract: | SUMMARY: A method of assessing the sporicidal efficiency of a UHT milk sterilizing plant operating on water is described. Water heavily contaminated with spores of a strain of Bacillus subtilis was filtered, after treatment in the plant, through membrane filters and the surviving spores estimated by incubation of the membranes in nutrient agar. With this plant a temperature of c . 135° caused a 99·99999% kill of B. subtilis spores. Confirmation of the lethal effects of temperatures above 135° was obtained by passing treated water into 10 gal churns containing sterile concentrated nutrient broth and incubating the churns. |
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