Source of cell injected is a critical factors for short and long engraftment in xeno-transplantation |
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| Authors: | G Noia M S Ligato E Cesari D Visconti G Fortunato M Tintoni I Mappa C Greco M E Caristo‡ G Bonanno§ M Corallo§ L Minafra§ A Perillo M Terzano† S Rutella§ G Leone§ G Scambia¶ M Michejda S Mancuso |
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| Institution: | Department of Obstetrics and Gynecology, Catholic University of the Sacred Heart, Rome, Italy,;Zootecnic Experimental Institute, Monterotondo, Italy,;Center for Experimental Animal Care, Catholic University of the Sacred Heart, Rome, Italy,;Hematology and Transfusion Service, Catholic University of the Sacred Heart, Rome, Italy,;Department of Oncology, Catholic University of the Sacred Heart, Campobasso, Italy, and;International Center for Intrdisciplinary Studies in Immunology, Georgetown University Medical Center, Washington, DC, USA |
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| Abstract: | Abstract. This study aims to investigate engraftment of human cord blood and foetal bone marrow stem cells after in utero transplantation via the intracoelomic route in the sheep. Here, we performed transplantation in 14 single and 1 twin sheep foetuses at 40–47 days of development, using a novel schedule for injection. (i) Single injection of CD34+ human cord blood stem cells via the coelomic route (from 10 to 50 × 104) in seven single foetuses. (ii) Single injection of CD34+ foetal bone marrow stem cells via the intracoelomic route with further numbers of cells (20 × 105 and 8 × 105, respectively) in three single and in one twin foetuses. (iii) Double fractioned injection (20–30 × 106) via the coelomic route and 20 × 106 postnatally, intravenously, shortly after birth of CD3-depleted cord blood stem cells in four single foetuses. In the first group, three single foetuses showed human/sheep chimaerism at 1, 8 and 14 months after birth. In the second group, the twin foetuses showed human/sheep chimaerism at 1 month after birth. In the third group, only two out of four single foetuses that underwent transplantation showed chimaerism at 1 month. While foetal bone marrow stem cells showed good short-term engraftment (1 month after birth), cord blood stem cells were able to persist longer in the ovine recipients (at 1, 8 and 14 months after birth). |
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