Molecular Characterization of a Genomic Fragment Containing Pi-kh Gene from the Genomic Library of Indica Rice Line Tetep |
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| Authors: | Maganti S Madhav Parikshit Plaha Nagendra K Singh and Tilak R Sharma |
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| Institution: | Authors' addresses: National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi 110012, India;;Present address: Directorate of Rice Research, Hyderabad 500030, India;;H.P.Agricultural University, Plamapur 176062, India (correspondence to T. R. Sharma. E-mail:;) |
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| Abstract: | Identification of full length genes along with upstream regulatory elements is important to understand its expression. Here, we report preparation of high titre genomic library and identification of a genomic clone containing Pi-k h gene with its complete upstream and downstream sequences from the rice blast resistant line Tetep. Structural analysis of protein revealed that Pi-k h has a central nucleotide binding site domain, leucine-rich repeats domain and a unique zinc-finger domain. Comparative analysis of Pi-k h protein sequence showed 64% and 45% similarity with the protein sequences of rice blast resistance genes Pi-b and Pi-ta , respectively. |
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| Keywords: | rice blast resistance gene NBS-LRR comparative analysis |
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