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PI3K/AKT and ERK regulate retinoic acid-induced neuroblastoma cellular differentiation |
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| Authors: | Jingbo Qiao Pritha Paul Sora Lee Lan Qiao Erlena Josifi Joshua R Tiao Dai H Chung |
| Institution: | a Department of Pathology, Dalhousie University, PO Box 15000, Halifax, Nova Scotia, Canada B3H 4R2 b Department of Microbiology & Immunology, Dalhousie University, PO Box 15000, Halifax, Nova Scotia, Canada B3H 4R2 c Department of Surgery, Dalhousie University, PO Box 15000, Halifax, Nova Scotia, Canada B3H 4R2 |
| Abstract: | Thymoquinone (TQ), a bioactive component of black caraway seed (Nigella sativa) oil, is reported to have antineoplastic properties. In this study we investigated the effect of TQ on a panel of human breast cancer cell lines. Cell viability assays showed that TQ killed T-47D, MDA-MB-231, and MDA-MB-468 cells via p53-independent induction of apoptosis; however, MCF-7 cells were refractory to the cytotoxic action of TQ. Western Blot analysis showed that MCF-7 cells expressed high levels of cytoprotective NADPH quinone oxidoreductase 1 (NQO1), which was responsible for TQ-resistance since inhibition of NQO1 with dicoumarol rendered MCF-7 cells TQ-sensitive. These findings may be clinically important when considering TQ as a possible adjunct treatment for breast cancer since a high percentage of breast tumors express NQO1. |
| Keywords: | Ab antibody DMSO dimethyl sulfoxide ERα estrogen receptor HER2 human epidermal growth factor receptor-2 HRP horseradish peroxidase MTT 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide NQO1 NADPH quinone oxidoreductase 1 PBS phosphate-buffered saline PI propidium iodide TQ thymoquinone |
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