一种新的脉冲电泳分子量标记*

在脉冲电泳（ｐｕｌｓｅｄ－ｆｉｅｌｄ　ｇｅｌ　ｅｌｃｔｒｏｐｈｏｒｅｓｉｓ，ＰＦＧＥ）研究中，经常使用的分子量标记有啤酒酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓ　ｃｅｒｅｖｅｓｌａｅ）和粟酒裂殖酵母（Ｓｃｈｉｚｏｓａｃｃｈａｒｏｍｙｃｅｓ　ｐｏｍｂｅ）的染色体完整ＤＮＡ。其中，啤酒酵母（如菌株ＹＮＮ２９５）有１６条染色体，分子量变化范围为０．２５Ｍｂ～２．２Ｍｂ（ＭｃＣｌｕｓｋｅｙｅｌａｌ，１９９０），适于作为小于２．２Ｍｂ的染色体ＤＮＡ的分子量标记；粟酒裂殖酵母（如菌株９７２ｈ－）有３条染色体，分子量…

A NOVEL DNA SIZE MARKER FOR MOLECULAR KARYOTYPEANALYSIS OF FUNGI

The molecular karyotypes of strain NJ7 and NJS of Debaryomyces hansenii were obscened byusing pulsed-field gel electrophoresis (PFGE) which employs contour-clamped homogeneous electric field (CHEF).Both strains were with & chromosomal DNAs. The eshmated chromosomal DNA sizes of strain NJ7 were 0.47Mb,0.89Mb, 1.05Mb, 1.36Mb, 2.20Mb, 2.20Mb, 2.80Mb, 3.05Mb, and srain NJS were 0.47Mb, 0.89Mb, 1.05Mb,1.36Mb, 2.05Mb, 2.20Mb, 2.80Mb, 3.05Mb, based on their migration relahve to the chromosomal DNAs ofSchizosacharomyces pombe and Saecharomyces cerevisiae, The genome sizes of strain NJ7 and NJ8 wereestimated to be 14. 02Mb and 13.87Mb respechvely. The results suggested that the molecular karyotype of stainNJ7 or NJ8 could be used as a new DNA size marker for molecular karyotype analysis of fungi.