Experimental design for the production of tensio-active agent by <Emphasis Type="Italic">Candida lipolytica</Emphasis> |
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Authors: | Raquel D Rufino Leonie A Sarubbo Benício B Neto Galba M Campos-Takaki |
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Institution: | Universidade Federal de Pernambuco, Recife-Pernambuco, CEP 50.670-420, Brazil. |
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Abstract: | The strategy of optimization using sequential factorial design was employed to enhance the tensio-active emulsifying agent
produced by Candida lipolytica using soybean oil refinery residue as substrate. A full factorial design was used to evaluate the impact of three fermentation
factors—amounts of refinery residue, glutamic acid and yeast extract. This allowed exclusion of the yeast extract. Full factorials
designs were then sequentially used to optimize the levels of the residue and glutamic acid. The surface tension value was
finally reduced to 25.29 mN/m. The maximum emulsifier activity using different substrates was within 40 h of cultivation.
The surface tension of the cell-free broth containing the biosurfactant remained very stable during exposure to a wide range
of pH (2–12), temperatures (0–120°C) and salinity (2–10% NaCl). The combination of an industrial waste and a cheap substrate
therefore seems to be very promising for the low-cost production of potent biosurfactant. |
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Keywords: | Medium optimization Candida lipolytica Biosurfactant Factorial design Refinery residue |
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