DNA structures contributing to the instability of recombinant plasmids inStreptococcus thermophilus |
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| Authors: | Daniel K. Y. Solaiman George A. Somkuti |
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| Affiliation: | (1) ARS, Eastern Regional Research Center, U.S. Department of Agriculture, 600 East Mermaid Lane, 19118 Philadelphia, PA, USA |
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| Abstract: | Summary Bifunctional shuttle vector (pBN183) and recombinant plasmids (pDCO2 and pDCO3) carrying aStreptomyces cholesterol oxidase gene (choA) were deleted to varying degrees inStreptococcus thermophilus. Restriction mapping of the deleted plasmids led to the identification of deletion prone regions in the transforming DNAs. Sequence analysis revealed that direct repeats and hairpin structures occurred in these regions, suggesting that they are deleterious to the stability of plasmids inS. thermophilus. |
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