Nuclear DNA content in species ofEleusine (Gramineae): A critical re-evaluation using laser flow cytometry |
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Authors: | K. S. Mysore V. Baird |
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Affiliation: | (1) Present address: Agronomy Department, Purdue University, West Lafayette, IN, USA;(2) Department of Horticulture, Clemson University, Poole Agricultural Bldg, Box 340375, 29634-0375 Clemson, SC, USA |
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Abstract: | Interest in dinitroaniline herbicide resistant biotypes ofEleusine indica, and an as yet undetermined taxon ofEleusine, necessitated a revaluation of reported nuclear genome size estimates for available species in the genus. Laser flow cytometry showed that the nuclear DNA content of six of the seven species examined had 15 to 50% less DNA than reported previously. It was also determined that roots, as contrasted to leaves, possessed a large fraction of nuclei at the 4C or 8C DNA content level, in diploid or tetraploid species, respectively (i.e. the G2/M peak). Two major reasons for the previously reported overestimation may include sampling only of root tissues where endopolyploid and normal diploid nuclei both occur and the inappropriate choice of onion nuclei as an internal standard. |
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Keywords: | Gramineae Eleusine indica E. multiflora E. coracana subsp.coracana E. coracana subsp.africana E. tristachya C-value genome size nuclear DNA nuclei isolation |
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