Functional characterization of thioredoxin h type 5 with antimicrobial activity from <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> |
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| Authors: | Seong-Cheol Park Young Jun Jung Il Ryong Kim Yongjae Lee Young-Min Kim Mi-Kyeong Jang Jung Ro Lee |
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| Institution: | 1.Department of Polymer Science and Engineering,Sunchon National University,Suncheon,Korea;2.National Institute of Ecology,Seocheon,Korea;3.Division of Applied Life Science (BK21+ Program) and PMBBRC,Gyeongsang National University,Jinju,Korea;4.Process Engineering R&D Center,Texas A&M University,College Station,USA |
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| Abstract: | A plant protein with effective antifungal activity was isolated from Arabidopsis thaliana leaves. The results of N-terminal sequencing analysis revealed that the isolated protein matches with a partial fragment of A. thaliana oxidoreductase thioredoxin h type 5; AtTrx-h5 (GenBank accession number AT1G45145). The AtTrx-h5 gene isolated from an Arabidopsis leaf cDNA library was transformed into Escherichia coli to investigate the functional characterizations of AtTrx-h5 protein. Recombinant AtTrx-h5 protein inhibited the conidia germination in 7 filamentous fungal cells and the proliferation in 3 pathogenic yeast cells. In addition, the cellular distribution of recombinant AtTrx-h5 protein in Fusarium solani showed pH-dependent cytosolic accumulation. Interestingly, AtTrx-h5 acted as an inhibitor of fungal growth via cellular reactive oxygen species (ROS) generation. We propose the novel functions of AtTrx-h5 as a potential candidate for natural antifungal material in the study. |
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