Comparison of optimal conditions for mass production of carboxymethylcellulase by Escherichia coli JM109/A-68 with other recombinants in pilot-scale bioreactor |
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| Authors: | Myung-Hwan Kim Wa Gao Chung-Han Chung Jin-Woo Lee |
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| Affiliation: | 1.Department of Medical Bioscience,Graduate School of Dong-A University,Busan,Korea;2.Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering,Hubei University of Technology,Wuhan,China;3.ReSEAT Programs,Korea Institute of Science and Technology Information,Seoul,Korea;4.Department of Biotechnology, College of Natural Resources and Life Science,Dong-A University,Busan,Korea |
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| Abstract: | The optimal conditions for mass production of carboxymethylcellulase (CMCase) by E. coli JM109/A-68 were investigated and compared with other E. coli JM109 recombinants producing CMCase. The optimal agitation speed and aeration rate for cell growth of E. coli JM109/A- 68 were 500 rpm and 0.50 vvm in a 7 L bioreactor, whereas those for production of CMCase were 416 rpm and 0.95 vvm. The optimal vessel pressures for cell growth as well as production of CMCase in a 100 L bioreactor were 0.04 MPa. The maximal production of CMCase by E. coli JM109/A-68 under the optimized conditions in a 100 L bioreactor was 11.0 times higher than its wild type, B. velezensis A-68. Optimal conditions for mass production of CMCase by recombinants were different from those for wild strains. The higher production of CMCase by E. coli JM109/A-68 and other recombinant of E. coli seemed to result from its higher cell growth under the optimal conditions for dissolved oxygen and its mixed-growth associated production pattern compared to the growthassociated production of B. velezensis A-68. |
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