The temperature sensitive mutant 72c |
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Authors: | Leif A. Isaksson and Renkichi Takata |
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Affiliation: | (1) Department of Molecular Biology, University of Uppsala, The Wallenberg Laboratory, Dag Hammarskjölds väg 21, S-752 37 Uppsala, Sweden;(2) Present address: Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-Cho, Hiroshima, Japan |
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Abstract: | Summary The spontaneous temperature sensitive mutant 72c is shown to be more tolerant to fusidic acid, but less tolerant to trimethoprim on plates at permissive temperature, than is the parental strain. The poor growth of the mutant on amino acids supplemented plates, as well as its inability to grow on broth plates at 40°, can be compensated by sublethal amounts of chloroamphenicol. Also some mutations to Rif-R or Str-R improve growth of the mutant under certain conditions.Reversion and other genetic analysis strongly suggest, that the pleiotropic behaviour of the mutant is due to a single mutation in a gene, which is designated fusB and is closely cotransducible with lip at min 14 of the E. coli chromosome. The gene order is lip-fusB-supE. |
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