Genetic Transformation in Methylococcus capsulatus

SUMMARY: Deoxyribonucleic acid (final concentration 100 μg/ml) extracted from wild-type cells of Methylococcus capsulatus was used to transform a p -amino benzoic acid (PABA) requiring strain (paba^-). Maximum levels of transformants were obtained towards the end of the exponential phase of growth in shake flask culture and the level of the population of transformants was not significantly altered by post-treatment incubation in enriched medium. The maximum transformation frequency was 0.17% of the initial population of PABA requiring cells and the back mutation frequency was 1 : 3.12 × 10⁸.