Synthesis of poly(adenosine diphosphate ribose) in synchronized Chinese hamster cells. |
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| Authors: | N A Berger A S Kaichi P G Steward R R Klevecz G L Forrest S D Gross |
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| Affiliation: | 1. Hematology/Oncology Division, Department of Medicine, Jewish Hospital of St Louis, Washington University, School of Medicine, St Louis, MO 63110, USA;2. Section of Cancer Biology, Division of Radiation Oncology, Mallinckrodt Institute of Radiology, Washington University, School of Medicine, St Louis, MO 63110, USA;3. Department of Cell Biology, Division of Biology, City of Hope National Medical Center, Duarte, CA 91010, USA |
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| Abstract: | Chinese hamster ovary cells were synchronized by mitotic selection and used to study the relation of poly(adenosine diphosphate ribose) synthesis to DNA synthesis and the different phases of the cell cycle. DNA synthesis was measured in cells rendered permeable to exogenously supplied nucleotides. Poly(ADPR) synthesis was also measured in permeable cells in the presence of both minimum and maximum DNA damage. The maximum DNA damage was produced by treating the cells with saturating concentrations of DNase. As anticipated, the DNA synthesis complex showed its maximum activity during S phase and showed 4–5-fold less activity during the other phases of the cell cycle. The basal level of poly(ADPR) synthesis was elevated during G1, fell to its lowest level during S phase, then increased during G2 and rose to its highest level during G1. The DNase responsive activity of poly(ADPR) synthesis was relatively constant thru the cell cycle but showed a peak at the end of S phase; then the activity decreased during the subsequent G2-M period. |
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