Sperm chromatin alterations in fertile and subfertile bulls |
| |
| Authors: | Elisson Terêncio Souza Cláudio Vieira Silva Bruno Augusto Nassif Travençolo Benner Geraldo Alves Marcelo Emílio Beletti |
| |
| Affiliation: | 1. Laboratory of Biology of Reproduction, Institute of Biomedical Sciences, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil;2. Faculty of Computer Science, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil |
| |
| Abstract: | Alterations in sperm chromatin have been related with subfertility in several mammals. In this study, chromatin alteration types (Base, Basal half, Central axis, Dispersed, and Whole) were assessed by toluidine blue (TB) staining, 6-diamidino-2-fenilindole (DAPI) and anti-protamine 1 antibody (anti-PR1) labeling in sperm samples of fertile and subfertile bulls. Semen samples were obtained from bulls kept in Artificial Insemination Center (fertile bulls) or from bulls subjected to scrotal insulation (subfertile bulls). The percentage of chromatin alterations identified by TB was similar (P?>?0.05) in semen samples of fertile and subfertile bulls. In contrast, a greater (P??0.05) among the methods In contrast, semen samples of subfertile bulls had a higher (P?
|
| |
| Keywords: | Computational image analysis DAPI Protamine Sperm chromatin Toluidine blue |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
