Optimization of culture conditions for 1,3-propanediol production from crude glycerol by <Emphasis Type="Italic">Klebsiella pneumoniae</Emphasis> using response surface methodology |
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Authors: | Baek-Rock Oh Jeong-Woo Seo Min Ho Choi Chul Ho Kim |
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Institution: | (1) Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580-185, Korea |
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Abstract: | To produce 1,3-propanediol (1,3-PD) from crude glycerol, cultivation conditions were optimized by response surface methodology
(RSM) based on a 25 factorial central composite design (CCD). RSM was adopted to derive a statistical model for the individual and interactive
effects of crude glycerol, (NH4)2SO4, pH, cultivation time and temperature on the production of 1,3-PD. Optimal conditions for maximum 1,3-PD production were
as follows: crude glycerol, 35 g/L; (NH4)2SO4, 8 g/L; pH, 7.37; cultivation time, 10.8 h; temperature, 36.88°C. Under these optimal conditions, the design expert presented
the maximal numerical solution with a predicted 1,3-PD production level of up to 13.74 g/L. The experimental production of
1,3-PD yielded 13.8 g/L, which was in close agreement with the model prediction. |
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Keywords: | central composite design crude glycerol 1 3-propanediol Klebsiella pneumoniae response surface methodology |
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