|
|||||
|
|
| 油菜蜂花粉抗氧化活性研究 | |
| 引用本文： | 赵剑喜,卜令娜,刘晔玮,裴栋,李丽,邸多隆. 油菜蜂花粉抗氧化活性研究[J]. 天然产物研究与开发, 2012, 24(7): 959-962 |
| 作者姓名： | 赵剑喜 卜令娜 刘晔玮 裴栋 李丽 邸多隆 |
| 作者单位： | 1. 兰州大学公共卫生学院营养与食品卫生研究所;中国科学院兰州化学物理研究所中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,兰州730000 2. 兰州大学公共卫生学院营养与食品卫生研究所 3. 中国科学院兰州化学物理研究所中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,兰州730000;中国科学院研究生院,北京100049 4. 中国科学院兰州化学物理研究所中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,兰州,730000 |
| 基金项目： | 甘肃省科技攻关项目，国家自然科学基金，甘肃省自然科学基金 |
| 摘 要： | 采用回流提取、大孔吸附树脂分离,得到8个油菜蜂花粉提取部位;利用H2O2诱导PC12细胞氧化损伤模型,评价各提取部位对PC12细胞氧化损伤的保护作用。在此基础上,采用HPLC法初步分析最佳抗氧化部位活性物质基础。结果表明,8个油菜蜂花粉提取部位中,部位B处理组细胞存活率最高,为90.0%,显著高于模型组53.6%(P<0.001),且高于VE阳性对照组85.0%(P<0.05)。通过对乙酸乙酯各提取部位进行HPLC分析,并用对照品标定色谱图中色谱峰,表明部位B含有3种主要黄酮:山奈酚-3,4’-双-O-β-D-葡萄糖苷(KMG)、山奈酚-3-O-β-D-(2-O-β-D-葡萄糖基)吡喃葡萄糖苷(KMP)和山奈酚-3-O-β-D-葡萄糖苷(KMC)。 |
| 关 键 词： | 油菜蜂花粉 抗氧化活性 PC12细胞 高效液相色谱(HPLC) 黄酮 |
Antioxidant Activity of Rape Bee Pollen |
|
| ZHAO Jian-xi , BU Ling-na , LIU Ye-wei , PEI Dong , LI Li , DI Duo-long. Antioxidant Activity of Rape Bee Pollen[J]. Natural Product Research and Development, 2012, 24(7): 959-962 | |
| Authors: | ZHAO Jian-xi BU Ling-na LIU Ye-wei PEI Dong LI Li DI Duo-long |
| Affiliation: | 1Institute of Nutrition and Food Hygiene of Public Health,Lanzhou University;2Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;3Graduate University of the Chinese Academy of Sciences,Beijing 100049,China |
| Abstract: | In this study,eight kinds of extract fractions were obtained from rape bee pollen using reflux and macroporous resin.And then antioxidant activity of eight kinds of extract fractions were evaluated using the H2O2-induced PC12 cells oxidatived model by MTT assays.The results showed that the survival rate of cells pretreated with fraction B reached 90.0%,significantly higher than that of the model group with 53.6%(P<0.001),and higher than that of the VE pretreated cells with 85.0%(P<0.05).Sequentially,main activity component in ethyl acetate extract fractions were speculated by HPLC assays and comparing chromatogram of the reference standard,it was found that the content of the three main flavonoids in fraction B,including kaempferol-3,4′-di-O-β-D-glucoside(KMG),kaempferol-3-O-β-D-(2-O-β-D-glucosyl)glucopyranoside(KMP) and kaempferol-3-O-β-D-glucoside(KMC),was distinctly higher than that of other fractions.This result implied that flavonoids was one of the main antioxidant active compounds in rape bee pollen.The results provide a reference for the utilization of rape bee pollen. |
| Keywords: | rape bee pollen antioxidant activity PC12 cell HPLC flavonoids |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |