Inhibition of cell proliferation and induction of apoptosis by genistein in experimental hepatocellular carcinoma |
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Authors: | Dechen Chodon S Mumtaz Banu R Padmavathi D Sakthisekaran |
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Institution: | (1) Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic, Medical Science, University of Madras, Taramani Campus, Chennai, 600 113, India |
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Abstract: | Hepatocellular carcinoma (HCC) is the leading cause of cancer related deaths in the world, with increasing incidence in many
developed countries. Epidemiological data suggest that consumption of soy products may be associated with a decreased risk
of cancer. We investigate the effects of genistein on cell proliferation, apoptosis and caspase-3 in DEN induced (200 mg/kg
body weight; by single intraperitoneal injection) and Phenobarbital promoted (0.05% through drinking water for 14 successive
weeks) cancer-bearing rats. Immunohistochemistry was employed to detect cell proliferating markers proliferating cell nuclear
antigen (PCNA), DNA fragmentation was determined by agarose gel electrophoresis and terminal deoxynucleatide transferase dUTP
nick labeling (TUNEL) staining and caspase by enzyme-linked immunosorbent assay. We found inhibition of cell proliferation,
induction of apoptosis and activation of caspase-3 in genistein treated animals. From these results, we conclude that genistein
inhibit cell proliferation, induced apoptosis. This activation of caspsase-3 in genistein treated liver cancer bearing animals
correlated well with its apoptosis inducing effect. |
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Keywords: | apoptosis cell proliferation caspase-3 genistein hepatocellular carcinoma |
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