Regulation of podosome formation in aortic endothelial cells vessels by physiological extracellular cues |
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| Affiliation: | 1. Université de Bordeaux, F-33000 Bordeaux, France;2. INSERM U1045, F-33000 Bordeaux, France;3. CBMN, UMR 5248, F-33000 Bordeaux, France;1. Department of Biology, Maynooth University, National University of Ireland, Maynooth W23F2H6, Co. Kildare, Ireland;2. Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth W23F2H6, Co. Kildare, Ireland;3. Institute of Physiology II, University of Bonn, D53115 Bonn, Germany;4. National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland;1. Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil;2. Laboratório de Inovações em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil;3. Laboratório de Imunoreceptores e Sinalização, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;4. Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil;5. Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil;6. Laboratório de Biologia Celular, Departamento de Biologia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil;1. Cell Physiology Laboratory, Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Shosse, 76a, 123007 Moscow, Russia;2. Faculty of Basic Medicine, Moscow State University, Lomonosovsky Prospekt, 31-5, 117192, Moscow, Russia;1. Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India;2. School of Biotechnology, University of Jammu, Jammu, 180006, India;3. Perelman School of Medicine, Cancer Biology Division, University of Pennsylvania, PA-19104, USA;4. Academy of Scientific & Innovative Research (AcSIR), CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India;5. Plant Biotechnology and System Biology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India;1. Department of Nanobiotechnology, Vision Research Foundation, Sankara Nethralaya Campus, Chennai, Tamil Nadu, India;2. School of Chemical and Biotechnology, SASTRA Deemed University, Tanjore, Tamil Nadu, India;3. Department of Cornea, Medical Research Foundation, Sankara Nethralaya Campus, Chennai, Tamil Nadu, India |
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| Abstract: | Invadosomes are specialised actin-based dynamic microdomains of the plasma membrane. Their occurrence has been associated with cell adhesion, matrix degrading and mechanosensory functions that make them crucial regulators of cell migration and invasion. Monocytic, cancer cell and Src-transformed cell invadosomes have been extensively described. Less well defined are the structures which form in other cell types, i.e., non-haematopoietic and non-transformed cells, exposed to specific stimuli. We herein describe the specificities of podosomes induced in aortic endothelial cells stimulated with TGFβ in vitro and in conditions that more closely resemble the in vivo situation. These podosomes display the typical architecture of monocytic podosomes. They organise into large rosette-shape superstructures where they exhibit collective dynamic behavior consisting in cycles of formation and regression. At the ultrastructural level, microfilament arrangements in individual podosomes were revealed. Oxygen levels and hemodynamic forces, which are key players in endothelial cell biology, both influence the process. In 3D environment, podosomes appear as globular structures along cellular extensions. A better characterization of endothelial podosomes has far-reaching implications in the understanding and, possibly, in the treatment of some vascular diseases. |
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| Keywords: | Endothelial cells Extracellular matrix Cytoskeleton Podosomes Vascular biology |
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